This study investigated the pharmacological significance of wild macrofungi collected from pine forests in Benguet and Mt. Province, Philippines, to help address a number of health-related conditions, such as oxidative stress, thrombosis, and bacterial infections. Six wild macrofungi were subjected to chloroform and ethanol extraction, and their crude extracts were screened for their total phenolic content (TPC), antioxidant, lethality, thrombolytic, and antibacterial activities. The ethanol extract of Daedaleopsis confragosa has the highest TPC at 49.28 ± 0.30 μg gallic acid equivalent (GAE)/mg extract and percent free radical inhibition activity at 74.59 ± 0.11%, which was comparable to the pure compound quercetin at 74.33 ± 0.32%. On the other hand, the ethanol extracts of Scleroderma citrinum and Postia fragilis have the most potent median effective concentration (EC50) at 431.01 ± 17.82 and 469.63 ± 15.25 μg/mL. Only the ethanol extract of Daedaleopsis confragosa exhibited low toxicity (median lethal concentration (LC50) = 565.90 μg/mL) while the rest of the test extracts are not toxic. Both chloroform and ethanol extracts of Termitomyces eurrhizus yielded the highest percent clot reduction values at 35.19 ± 0.13% and 32.41 ± 0.17%, respectively. This is the first study to report the thrombolytic activity of macrofungi extracts from the Philippines. The ethanol extract of Lenzites betulina gave the highest zone of inhibition (ZOI) against Staphylococcus aureus American Type Culture Collection (ATCC) 25923 (13.33 ± 0.58 mm) while the chloroform extract of Daedaleopsis confragosa gave the highest ZOI against Escherichia coli ATCC 25922 (12.33 ± 1.15 mm). These data indicated that the wild macrofungi tested could constitute a potential source of natural bioactive compounds in the production of pharmaceutical dosage forms or nutraceuticals.
Keywords: macrofungi, total phenolic content, antioxidant, lethality, thrombolytic, antibacterial, PhilippinesAnticancer Compounds From Nine Commercially Grown and Wild Philippine Mushrooms
Ajith, T.A., & Janardhanan, K.K. (2007). Indian medicinal mushrooms as a source of antioxidant and antitumor agents. Journal of Clinical Biochemistry and Nutrition, 40, 157–162.
Ao, T., Deb, C.R., & Khruomo, N. (2016). Wild edible mushrooms of Nagaland, India: A potential food resource. Journal of Experimental Biology and Agricultural Sciences, 4(1), 59–65.
Barros, L., Venturini, B.A., Baptista, P., Estevinho, L.M., & Ferreira, I.C.F.R. (2008). Chemical composition and biological properties of Portuguese wild mushrooms: A comprehensive study. Journal of Agricultural and Food Chemistry, 56(10), 3856–3862.
Berch, S.M., Ka, K.-H., Park, H., & Winder, R. (2007). Development and potential of the cultivated and wild-harvested mushroom industries in the Republic of Korea and British Columbia. BC Journal of Ecosystems and Management, 8(3), 52–75.
Cheung, P.C.K. (2013). Mini-review on edible mushrooms as source of dietary fiber: Preparation and health benefits. Food Science and Human Wellness, 2, 162–166.
Clarkson, C., Maharaj, V.J., Crouch, N.R., Grace, O.M., Pillay, P., Matsabisa, M.G., Bhagwandin, N., Smith, P.J., & Folb, P.I. (2004). In vitro antiplasmodial activity of medicinal plants native to or naturalized in South Africa. Journal of Ethnopharmacology, 92, 177–191.
Clinical and Laboratory Standards Institute. (2017). Performance standards for antimicrobial susceptibility testing (27th ed.) PA, USA: Clinical and Laboratory Standards Institute.
Ferreira, I.C.F.R., Baptista, P., Vilas-Boas, M., & Barros, L. (2005). Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chemistry, 100, 1511–1516.
Gan, C.H., Nurul Amira, B., & Asmah, R. (2013). Antioxidant analysis of different types of edible mushrooms (Agaricus biporous and Agaricus brasiliensis). International Food Research Journal, 20(3), 1095–1102.
Gaylan, C.M., Estebal, J.C., Tantengco, O.A., & Ragragio, E. (2018). Anti-staphylococcal and antioxidant properties of crude ethanolic extracts of macrofungi collected from the Philippines. Pharmacognosy Journal, 10(1), 106–109.
Ginns, J. (2017). Polypores of British Columbia (Fungi: Basidiomycota). Victoria, BC: Province of British Columbia.
Ginns, J. (2019). Annotated key to Pacific Northwest polypores. Victoria, BC: Pacific Northwest Key Council.
Gonthier, P., & Nicolotti, G. (2007). A field key to identify common wood decay fungal species on standing trees. Arboriculture & Urban Forestry, 33(6), 410–420.
Griffin, J.P. (Ed.). (2009). The textbook of pharmaceutical medicine (6th ed.). Oxford, UK: Wiley-Blackwell.
Guevara, B.Q. (Ed.). (2004). A guidebook to plant screening: Phytochemical and biological. Manila, Philippines: Research Center for the Natural Sciences, University of the Santo Tomas.
Ho, L.-H., Zulkifli, N.A., & Tan, T.-C. (2020). Edible mushroom: Nutritional properties, potential nutraceutical values, and its utilization in food product development. In A.K. Passari & S. Sanchez (Eds.), An introduction to mushrooms (pp. 19–38). Germany: BoD. doi: 10.5772/intechopen.91827
Hussein, J.M., Tibuhwa, D.D., Mshandete, A.M., & Kivaisi, A.K. (2015). Antioxidant properties of seven wild edible mushrooms from Tanzania. African Journal of Food Science, 9(9), 471–479.
Keleş, A., Koca, I., & Genҫcelep, H. (2011). Antioxidant properties of wild edible mushrooms. Journal of Food Processing and Technology, 2(6). doi: 10.4172/2157- 7110.1000130
Kirk, P.M., Cannon, P.F., Minter, D.W., & Stalpers, J.A. (2008). Ainsworth and Bisby’s dictionary of the fungi (10th ed.). Wallingford: CABI.
Kouassi, K.P., Kouadio, E.J.P., Konan, K.H., Due, A.E., & Kouame, L.P. (2016). Phenolic compounds, organic acids and antioxidant activity of Lactarius subsericatus, Cantharellus platyphyllus and Amanita rubescens, three edible ectomycorrhizal mushrooms from the center of Cote d’Ivoire. Eurasian Journal of Analytical Chemistry, 11(3), 127–139.
Lallawmsanga, Passari, A.K., Mishra, V.M., Leo, V.V., Singh, B.P., Meyyappan, G.V., Gupta, V.K., Uthandi, S., & Upadhyay, R.C. (2016). Antimicrobial potential, identification and phylogenetic affiliation of wild mushrooms from two sub-tropical semi-evergreen Indian forest ecosystems. PLoS ONE, 11(11), e0166368.
Licyayo, C.M. (2018). Gathering practices and actual use of wild edible mushrooms among ethnic groups in the Cordillera, Philippines. In A. Niehof, H.N. Gartaula, & M. Quetulio-Navarra (Eds.), Diversity and change in food wellbeing—cases from Southeast Asia and Nepal (pp. 71–86). The Netherlands: Wageningen Academic Publishers.
Liu, Z.-G., Bao, L., Liu, H.-W., Ren, J.-W., Wang, W.-Z., Wang, L., Li, W., & Yin W.-B. (2017). Chemical diversity from the Tibetan Plateau fungi Penicillium kongii and P. brasilianum. Mycology, 9(1), 10–19.
Lu, C.-L., & Chen, S.-N. (2012). Fibrinolytic enzymes from medicinal mushrooms. In E. Faraggi (Ed.), Protein structure (pp. 337–362). Shanghai, China: In Tech.
Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., & McLaughlin, J.L. (1982). Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica, 45, 31–34.
Molyneux, P. (2004). The use of the stable free radical diphenylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.
Mortimer, P.E., Xu, J., Karunarathna, S.C., & Hyde, K.D. (Eds.). (2014). Mushrooms for trees and people: A field guide to useful mushrooms of the Mekong region. Kunming, China: World Agroforestry Center, East and Central Asia.
Obodai, M., Ferreira, I.C.F.R., Fernandes, A., Barros, L., Mensah, D.L.N., Dzomeku, M., Urben, A.F., Prempeh, J., & Takli, R.K. (2014). Evaluation of the chemical and antioxidant properties of wild and cultivated mushrooms of Ghana. Molecules, 19, 19532–19548.
Ogidi, O.C., & Oyetayo, O. (2016). Phytochemical property and assessment of antidermatophytic activity of some selected wild macrofungi against pathogenic dermatophytes. Mycology, 7(1), 9–14 . doi: 10.1080/21501203.2016.1145608
Okhuoya, J.A., Akpaja, E.O., Osemwegie, O.O., Oghenekaro, A.O., & Ihayere, C.A. (2010). Nigerian mushrooms: Underutilized non-wood forest resources. Journal of Applied Sciences and Environmental Management, 14(1), 43–54.
Ostry, M.E., Anderson, N.A., & O’Brien, J.G. (2011). Field guide to common macrofungi in eastern forests and their ecosystem functions. PA, USA: U.S. Forest Service.
Patowary, B.S. (2010). Mushroom poisoning—an overview. Journal of College of Medical Sciences–Nepal, 6(2), 56–61.
Prasad, S., Kashyap, R.S., Deopujari, J.Y., Purohit, H.J., Taori, G.M., & Daginawala, H.F. (2006). Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thrombosis Journal, 4, 14.
Ramawat, K.G., & Merillon, J.-M. (2013). Natural products: Phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes. Heidelberg, Germany: Springer-Verlag.
Rodwell, V.W., Bender, D.A., Botham, K.M., Kennelly, P.J., & Well, P.A. (2015). Harper’s illustrated biochemistry (30th ed.). NY, USA: McGraw Hill Education.
Subbulakshmi, M., & Kannan, M. (2016). Cultivation and phytochemical analysis of wild mushrooms Daldinia concentrica and Pheolus schweinitzii from Tamilnadu, India. European Journal of Experimental Biology, 6(3), 46–54.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia, 1(1), 98–106.
Toledo, C.V., Barroetavena, C., Fernandes, A., Barros, L., & Ferreira, I.C.F.R. (2016). Chemical and antioxidant properties of wild edible mushrooms from native Nothofagus spp. forest, Argentina. Molecules, 21(9), 1201. doi: 10.3390/molecules21091201
Tripathy, S.S., Rajoriya, A., Mahapatra, A., & Gupta, N. (2016). Biochemical and antioxidant properties of wild edible mushrooms used for food by tribes of eastern India. International Journal of Pharmacy and Pharmaceutical Sciences, 8(4), 194–199.
Valverde, M.E., Hernandez-Perez, T., & Paredes-Lopez, O. (2015). Edible mushrooms: Improving human health and promoting quality life. International Journal of Microbiology, 2015, 376387. doi: 10.1155/2015/376387
Wasser, S.P., & Weis, A.L. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms: Current perspective. International Journal of Medicinal Mushrooms, 1, 31–62.
Waterhouse, A.L. (2002). Determination of total phenolics. Current protocols in food analytical chemistry. NY, USA: John Wiley & Sons, Inc.
Wong, F.-C., Chai, T.-T., Tan, S.-L., & Yong, A.-L. (2013). Evaluation of bioactivities and phenolic content of selected edible mushrooms in Malaysia. Tropical Journal of Pharmaceutical Research, 12(6), 1011–1016.
World Health Organization. (2019). World health statistics 2019: Monitoring health for the SDGs. Geneva: World Health Organization.
Yildirim, N.C., Turkoglu, S., Yildirim, N., & Ince, O.K. (2012). Antioxidant properties of wild edible mushroom Pleurotus eryngii collected from Tunceli Province of Turkey. Digest Journal of Nanomaterials and Biostructures, 7(4), 1647–1654.